Rapid Multiplication of Dendrocalamus hamiltonii in vitro Regeneration Techniques from Nodal Explants

The present study reported an advanced, effective and reproducible in vitro propagation protocol for Dendrocalamus hamiltonii using nodal explants. Secondary branches of the mother plant of D. hamiltonii were used to collect nodal explants of length 12-15 mm. Explant sterilization was carried out with 70% ethyl alcohol for 30s followed by mercuric chloride (0.1%) for 5 min and then inoculated on MS basal media for establishment. For multiplication, established shoots were implanted on MS media supplemented with 4.0 mg/l BAP, which resulted in a greater number of shoots multiplication after 21 days of incubation. Clumps of excised propagules transplanted on ½ MS media supplemented with 3 mg/l IBA + 3 mg/l IAA and 2 % sucrose induced rooting after 25 days and profuse rooting of shoots after 40 days of incubation. A two-step acclimatization process was done, in which soil: sand: vermicompost (1:1:1) media showed 80 per cent survival in in vitro raised shoots of D. hamiltonii. Field-transplanted plants exhibit prolific growth and development. This study was carried out to standardise multiplication of Dendrocalamus hamiltonii through In vitro regeneration.