Effect of Storage Containers and Media on Germination of Dysoxylum malabaricum Bedd. In Hilly Zone of Karnataka
Abstract
The primary objective of seed storage is to preserve viability with minimal loss. However, ex-situ storage of recalcitrant seeds is challenging, as they lose viability within days to a few months due to dehydration. This study aimed to extend seed longevity of Dysoxylum malabaricum, an endangered evergreen tree, by evaluating different storage containers and treatments. Seeds collected from natural populations were stored in three containers-polythene bags, poly pots and earthen pots-under seven treatments: control, carbendazim (0.5%), chlorpyrifos (2%), abscissic acid (0.005%), carbendazim (0.5%) + chlorpyrifos (2%), carbendazim (0.5%) + chlorpyrifos (2%) + abscissic acid (0.005%), and cow dung + ash. Poly pots were the most effective, and maintained viability for 45 days with 63.18% germination. Among remaining treatments, Chlorpyrifos (2%) was superior, retaining viability for 45 days with 94.81% germination. The highest germination (97.78%) was in seeds stored in polythene bags treated with chlorpyrifos (2%) for 45 days. Appropriate storage treatments and container selection can thus significantly prolong the viability of D. malabaricum seeds, aiding the conservation and sustainable use of this endangered species.
Keywords
Dysoxylum malabaricum
Recalcitrant seeds
Seed viability
Storage containers
Germination