Ameliorative Potential of Wheatgrass against Histological and Biochemical Alterations caused due to Chlorantraniliprole and Arsenic in Female Albino Rats

The ameliorative potential of wheatgrass juice was evaluated against the reproductive toxicity caused by combination of pesticide, chlorantraniliprole and heavy metal, arsenic in the female albino rats to address the growing concerns about simultaneous exposure to agrochemicals and heavy metals in the environment and assess their impact on female reproductive function. The rats were administered with chlorantraniliprole (1/2, 1/4 and 1/6 LD50), arsenic (0.50 mg/L of water) and fresh wheatgrass juice (10 ml/kg b.w.) orally. The significant alterations such as increase in duration of estrous cycle, reduced number of follicles, increased atresia (%) in the ovaries, decreased diameter of uterine epithelium, myometrium and endometrium and scattering of lumen in the uterus; reduction in the protein and lipid content were observed in chlorantraniliprole (1/2, 1/4 LD50) + arsenic treated rats as compared to control rats. However, less significant alterations were observed in the reproductive parameters of the rats treated with groups exposed to chlorantraniliprole (1/6 LD50) + arsenic and only arsenic when compared to the control group. These changes were reduced with administration of fresh wheatgrass juice thus suggesting that wheatgrass acts as an ameliorative agent against chlorantraniliprole and arsenic induced female reproductive toxicity rats.